Composite
Part:BBa_K2717010:Design
Designed by: Chenyu Liu Group: iGEM18_BNU-China (2018-10-09)
gdh-gfp-reverse
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1157
Illegal AgeI site found at 2049 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 644
Illegal BsaI.rc site found at 1175
Design Notes
NONE
Source
The Promoter J23100, RBS 2000 and GFP are derived from the Kit. The glucose dehydrogenase is isolated from the genome of the laboratory strain and V5 tag and His tag are obtained from our own lab.